منابع مشابه
2-ISleto-4-hydroxyglutarate Aldolase of Bovine Liver
Z-Keto-4-hydroxyglutarate aldolase, which catalyzes the reversible cleavage of Z-keto-4-hydroxyglutarate to yield pyruvate and glyoxylate, has been purified 1300-fold from bovine liver extracts. The purified enzyme appears to be homogeneous when examined by electrophoresis on starch gel or cellulose polyacetate (Sepraphore III) or by chromatography on columns of Sephadex. A molecular weight of ...
متن کاملThe carboxyl-terminal structure of rabbit liver aldolase (aldolase B).
The amino acid residues in the carboxyl-terminal region of fructose diphosphate aldolase isolated from fresh rabbit liver are -(Gly,Phe,Leu,Ala,Thr2,Ser2)-Tyr. This differs from the carboxyl-terminal structure in aldolase isolated from tissue which had been frozen and thawed. Amino acid analyses, ultracentrifugation, and digestion with carboxypeptidase suggest that the difference is the result ...
متن کاملDeoxyribose Phosphate Aldolase from Rat Liver.
The activity and properties of deoxyribose phosphate aldolase from Escherichia coli and Lactobacillus plantarum have been described in detail by Racker (1) and Pricer and Horecker (2). The enzyme from E. coli was shown to be active at pH values between 6.0 and 8.0 in tris(hydroxymethyl)aminomethane and phosphate buffers without exhibiting a sharp optimum (1). The enzyme from L. plunturum was sh...
متن کاملSulfhydryl groups in relation to the structure and catalytic activity of 2-oxo-4-hydroxyglutarate aldolase from bovine liver.
Bovine liver 2-oxo-4-hydroxyglutarate aldolase (suggested name: 2-oxo-4-hydroxyglutarate glyoxylate-lyase catalyzing the reaction: 2-oxo-4-hydroxyglutarate in equilibrium pyruvate + glyoxylate) contains eight to ten sulfhydryl groups as determined by titration of the enzyme with either 5,5'-dithiobis(2-nitrobenzoic acid) (Nbs2) or p-mercuribenzoate in the presence of 1% sodium dodecyl sulfate. ...
متن کاملInactive enzyme molecules in aging mice: liver aldolase.
Evidence is presented that there is a considerable accumulation of inactive fructose-1,6-diphosphate aldolase (EC 4.1.2.7) in the liver of senescent mice. Liver aldolase was purified from 3-month-old mice and used to immunize rabbits. It was demonstrated with the monospecific antibody thus produced that the liver aldolase of young adult (3 month) and aged (31 month) mice are antigenically ident...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64766-0